cy5 nhs ester  (MedChemExpress)

Journal: The Journal of Biological Chemistry

Article Title: EGFR-targeted gadolinium contrast agents for enhanced molecular magnetic resonance imaging of tumors

doi: 10.1016/j.jbc.2025.110992

Figure Lengend Snippet: EBP-Gd-DO3A conjugates: structures and cellular gadolinium. Schematic structures of ( A ) EBP-Gd-DO3A; ( B ) EBP-(Gd-DO3A) 3 ; and ( C ) Cy5.5-EBP-Gd-DO3A. And cells were incubated with EBP-Gd-DO3A, EBP-(Gd-DO3A) 3 , or Gadovist at a Gd concentration of 50 μM for 1 h ( D ) or 12 h ( E ) at 37 °C. The intracellular Gd content was quantified by ICP–MS. Bars represent the mean ± SD. The data represent three independent experiments. Statistical significance was evaluated using one-way ANOVA followed by Tukey’s post hoc test. ∗ p < 0.05, ∗∗ p < 0.01,∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Cy5.5 N-Hydroxysuccinimide ester (Cy5.5-NHS) was purchased from Lumiprobe (Hallandale Beach, FL).

Techniques: Incubation, Concentration Assay

Journal: The Journal of Biological Chemistry

Article Title: EGFR-targeted gadolinium contrast agents for enhanced molecular magnetic resonance imaging of tumors

doi: 10.1016/j.jbc.2025.110992

Figure Lengend Snippet: Cellular Gd accumulation of contrast agents in tumor cells. MDA-MB-231, A549, SGC-7901, SW480, and U138 MG cells were incubated with EBP-Gd-DO3A, EBP-(Gd-DO3A) 3 or Gadovist for 1 h ( A ) and 12 h ( B ). The cellular Gd contents were determined by ICP-MS and are presented as 50 nM Gd/10 6 cells. The data are presented as the mean ± SD of 6 samples for each cell line. For statistical analysis, Mann-Whitney test was used for ( A ) and ( B ). ∗ p < 0.05; ∗∗ p < 0.01 (with vs without C225 pretreatment). Confocal fluorescence microscopy images displaying the cellular localization of the Cy5.5 ( C ) and Cy5.5-EBP-Gd-DO3A ( D ) in MDA-MB-231 and U138 MG cells after 1 h of exposure to the agent. The cells were treated with Cy5.5 or Cy5.5-EBP-Gd-DO3A for 1 h and visualized under a fluorescence microscope. Binding and endocytosis of the Cy5.5-labelled EBP-Gd-DO3A conjugate in MDA-MB-231 and U138 MG cells and inhibition of binding by C225. The cells were preincubated with C225 for 12 h and then treated with equimolar concentrations of Cy5.5 ( E ) or Cy5.5-EBP-Gd-DO3A ( F ) for 1 h prior to visualization. Fluorescence microscopy was performed. Merged images display the overlay of nuclear DAPI staining ( blue ) and Cy5.5 dye ( red ).

Article Snippet: Cy5.5 N-Hydroxysuccinimide ester (Cy5.5-NHS) was purchased from Lumiprobe (Hallandale Beach, FL).

Techniques: Incubation, MANN-WHITNEY, Fluorescence, Microscopy, Binding Assay, Inhibition, Staining